FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318051

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318051
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3501915
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC51420.14683394656923426No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC51370.14669116754689934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT31550.048.255459
TGGCGCG32250.047.42262315
CGCGGTG36200.042.43922410
CTACTCG38450.039.812137
TACTCGG39250.039.01294738
GTAGTCC41050.037.73079327
CCGAACT6800.037.38292767
ACTCGGG42850.036.64085439
AGGATCG41650.036.38542659
TAGTCCC42750.036.315628
CGTGCCT42850.036.19355420
GATCGCT42600.036.18729861
CGGCATA2800.036.0607341
GCCGGTT7350.035.7787743
TGTAGTC43200.035.7661326
CGAACTT7600.035.52153468
GGGGGGA66050.034.9490431
GCGGTAT2050.033.968061
ATCGCTT45650.033.78565262
CGGCAAT3850.033.4607471
Produced by FastQC (version 0.11.5)