FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493856
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG154120.4411172068911827No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG54590.1562457067492192No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG39310.11251179212881125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA41100.035.44265432
GCGGCAC43150.033.92395433
CGGCACC44150.033.00459734
TCGCCGG43400.031.87083448
CCCGTCG44750.030.94306444
CCGTCGC44950.030.89301345
CGTACGT2150.030.59623319
CCCCCGA50300.029.32205619
ACCCGCG50350.029.24302725
GCACCCC51250.028.7905736
AGTCCGC54150.027.9945147
CACCCCC53000.027.97778337
CCCCGAC53000.027.8322820
CAGTCCG53650.027.2232536
CCGCGCG55400.026.52167927
TCCGCCC58300.026.4763059
GGCACCC55850.026.46965635
GTCCGCC57650.026.4146258
CCCGACC56950.025.96377821
CCCCCGT54750.025.33812942
Produced by FastQC (version 0.11.5)