FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3493856
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC50960.1458560398596851No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC47360.13555223798576702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT32800.046.4234479
TGGCGCG35300.042.83603315
CGCGGTG38250.040.07971610
CTACTCG38700.038.5705737
TACTCGG39100.038.0970638
CCGAACT5850.037.44213567
GTAGTCC41650.036.2621427
AGGATCG40900.035.33336359
ACTCGGG43200.035.2917339
CGTGCCT43150.034.8952720
GATCGCT42200.034.53717861
TAGTCCC43650.034.52623428
CGGGTAG6150.034.045461
TGTAGTC45000.033.55973426
CGGCAAA7850.033.34071
GCGTGCC45800.033.32538619
CGGCATA3050.032.036221
CGGTTCA7300.031.7631035
CGGTAGA3850.031.7241821
CCTGTAG47750.031.68951424
Produced by FastQC (version 0.11.5)