FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3372959
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG201320.5968646520755218No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG134730.39944155858402075No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132100.3916442506416473No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG83120.24643050804946046No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC82240.24382152288243056No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT79710.23632069052721957No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT66740.1978678068722448No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC62260.18458570056736534No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA60460.17924913999844055No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG56020.16608562392842605No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54120.16045258777233878No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT50140.14865285940327172No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA48300.14319770859948194No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG46030.13646771277089345No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT43450.12881864262210124No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG39590.11737468495762919No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG39390.11678173378330421No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC37130.11008138551343197No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC34410.10201724954261228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA58350.036.6188332
GCGGCAC59400.035.98990233
CGGCACC61250.034.9767634
CGTCGTT905.4169504E-934.3531573
TCGCCGG60050.034.10848248
CCGTCGC60400.033.7759745
CCCGTCG61550.033.28693444
GCACCCC67950.031.66999636
CCCCCGA68600.031.52831819
TCGTATG2650.031.52591140
CACCCCC70400.030.54519837
GGCACCC70750.030.39878835
TATCTCG2750.030.2917536
AGGACCG6350.029.99035370
ACCCGCG72250.029.87961425
CTTACCC12500.029.6683570
CCCCGAC74350.029.00962820
CGTACGT2050.028.51473819
TCGTATC3100.027.94217733
CCCCCGT73400.027.58840842
Produced by FastQC (version 0.11.5)