FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358232
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG203570.6061820624662024No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG133590.3977986035509161No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN129060.3843093627837505No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG81320.2421512271933565No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC80970.2411090121230457No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT78360.23333706545587085No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT64830.1930480085949988No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC64660.19254178984656212No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA60460.18003520900283246No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG55700.1658610840466055No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG53590.1595780160513032No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT51100.15216340026537772No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG47020.14001415030289746No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA46460.1383466061904002No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT42490.1265249095357319No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG39120.1164898672873107No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG38200.11375033053106515No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36640.1091050290748227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA56600.037.1333532
GCGGCAC58950.035.73604233
TCGCCGG57200.034.90249348
CGGCACC61050.034.3593434
CCCGTCG59400.033.97219544
CCGTCGC59400.033.76635445
GTTCCGT2850.032.8107071
GCACCCC65800.031.92092136
CCCCCGA67500.031.011419
GGCACCC69750.030.34144435
CACCCCC69550.030.27444337
ACCCGCG68900.030.21596125
GTGCCGT5150.029.5898081
TATCTCG2000.029.50970636
CCCCGAC70900.029.4422820
CCCCCGT70950.028.35426142
GATAGGC25300.027.7941740
CTTACCC12850.027.74858770
TACGGGT18200.027.723196
CGTACGT1750.027.49779519
Produced by FastQC (version 0.11.5)