FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3358232
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125900.3748996495775158No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA125450.3735596587728305No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76990.22925753789493994No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC64670.19257156741999956No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA56000.16675441124972903No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG38930.11592409339199912No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC37070.11038546473263312No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36640.1091050290748227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT40550.048.4140479
TGGCGCG42400.046.30164315
GGACTCT46400.045.42363
ACTCTTC46950.045.2570465
GGGGACT44500.044.780021
TCTGGTC47350.044.28814310
TCTACGG3200.043.980995
GGGACTC48050.043.4461942
TTCTGGT48750.043.4386949
CGCGGTG45700.043.03330210
TGGTCCG15500.042.0708812
CCGAACT6650.041.943267
CTGGTCC50000.041.94087211
GTAGTCC48750.040.7703327
CTACTCG48600.040.67421337
CGGGACT10950.040.370521
TACTCGG49150.040.31352638
TACGGCC3550.039.6412857
GACTCTT56400.039.3782424
TGTAGTC50650.039.215626