FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366895
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG204840.6083943811731581No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG131410.39030026181392646No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128880.3827859199648341No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC83400.24770597241672224No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG81270.24137966880464048No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT79150.23508306614848398No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT66420.1972737492556198No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC63330.18809615387471246No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA61350.1822153646015097No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG57800.1716715252480401No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG54720.16252363082305804No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT52190.15500928897396563No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA48430.14384172954606544No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG46580.13834705269989114No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT41640.12367478047280953No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG39100.11613073766779183No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG38360.11393286692932211No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36340.1079332738324183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA60750.037.83343532
GCGGCAC62750.036.4911733
CGGCACC64500.035.57835434
TCGCCGG62150.035.4924648
CCGTCGC63850.034.62302845
CCCGTCG64400.034.36176344
GCACCCC67900.033.88816536
CCCCCGA71150.032.6661819
ACCCGCG71350.032.3270725
GGCACCC71800.032.07451235
CACCCCC73450.031.39937637
CCCCGAC75200.030.92259420
TAGTCCG4050.030.5108956
GATAGGC26350.029.72916840
CCCCCGT75100.029.52093742
GCATTAG27050.028.93220512
CATTAGC27600.028.60439313
CGTCGCC78100.028.28335446
CCGCGCG81900.028.23282827
AGTCCGC82500.028.1671077
Produced by FastQC (version 0.11.5)