FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352624
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG204320.6094330888283327No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG133150.3971516042359656No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN128520.3833415259211889No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG82930.24735848696424054No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC82760.2468514214537628No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT77900.23235531333069262No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT65220.19453419172564534No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC64950.1937288523854748No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA59560.17765189296503275No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG57570.17171624375414601No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG56170.16754041013844678No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT51140.15253723650489887No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA48140.1435890216141148No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG47740.1423959262953436No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT42790.1276313717255499No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG40030.11939901402602857No Hit
GGGGTGAACTCGGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTG39090.11659524002691622No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC35470.1057977273920368No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC34440.10272550694620095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGACT350.001380125639.2412072
TTACGCG350.001382972739.2247615
CGCGGCA58250.037.909632
GCGGCAC59000.037.39048433
CGGCACC61400.035.95177534
TATCGGA1650.035.363838
TCGCCGG59850.035.05082348
CCGTCGC60700.034.52382745
TACGCGA400.002663883834.3216616
CCCGTCG61800.033.9410244
TATCTCG1850.033.7655736
TTATCGG1750.033.343047
GCACCCC67000.033.1496236
CCCCCGA68450.032.74059319
ACCCGCG69350.032.10495425
GTTATCG1950.031.683436
CACCCCC70250.031.63899237
TACGGGT20650.031.5811446
GGCACCC71350.031.0022135
CCCCGAC72800.030.65685720
Produced by FastQC (version 0.11.5)