FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352624
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN125260.3736177990732036No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA120740.3601358219710889No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76390.2278513785023313No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC62980.18785285794052659No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA53740.1602923560769117No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC36420.10863132877411842No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG35710.10651358458329953No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT34630.10329222722261727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT38550.050.2128269
CCGAACT6250.047.52542567
TGGCGCG41300.047.11513515
GGGGACT41750.045.825671
CGCGGTG43750.044.24467510
GGACTCT45650.044.202263
ACTCTTC45250.044.1439175
TCTGGTC44950.043.9797510
TGGTCCG13700.043.8380712
GGGACTC49200.041.5147932
CTACTCG47400.041.45800837
TTCTGGT47850.041.3143129
TACTCGG47800.041.2086838
GTAGTCC49200.040.6606527
CGGGACT12100.040.315671
TAGTCCC49550.040.11975528
CTGGTCC49600.039.92584211
GCCGGTT7600.039.7353483
CGTGCCT50950.039.0717120
TGTAGTC51200.038.91827826