FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159411
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG143780.45508482435491937No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG80830.25583882565452865No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC68600.21712907880614457No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC55980.17718492465842525No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG48440.1533197168712776No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG47920.15167384047216395No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT47480.15028117582676012No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG40240.12736551211602415No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37800.11964255362787557No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35250.11157142897837605No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34900.1104636275558957No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC33730.10676040565789004No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC33090.10473471162821171No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT31870.10087323238413744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA44450.036.46572532
GCGGCAC45500.035.5554433
CGGCACC47000.034.576934
CCTATCG501.8598264E-434.5073432
GTTCCGT2750.033.980611
TCGCCGG47500.032.5268848
GCACCCC50350.032.36473536
CCGTCGC48100.032.0615845
CCCGTCG48100.031.82462344
GTGCCGT5400.030.7643381
GGCACCC53300.030.6947535
CCCCCGT50500.030.14116542
CGCCCTA14200.030.0506861
CCCCCGA53850.030.00630619
CTTACCC11650.029.91270370
CGTACGT2450.029.59413319
GTTATCG2800.029.5777236
CGATCTA2550.028.7079947
CACCCCC57300.028.69125437
TCGTTTG18700.028.60732817
Produced by FastQC (version 0.11.5)