FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3159411
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC116930.3701006295160712No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC102880.3256303152707894No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA73020.23111902819861044No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT59060.18693357717625214No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC44610.1411972041624214No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC38100.12059209770428729No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34730.1099255525792624No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34380.10881775115678208No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG31680.10027185446907667No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT55250.052.1955389
CCGAACT9800.050.5188167
CGCGGTG57550.050.35088310
TGGCGCG58800.049.51595315
GTCTACG5750.048.592554
CGGTTCA9900.048.4319655
TACGGCC6300.045.445417
GCCGGTT10900.044.3100433
TCTACGG6400.044.1963465
CGAACTT12250.042.30116768
CCGGTTC11450.042.1769374
TAGTCCC71150.041.55914328
CCGACTC8750.041.398243
GTAGTCC71700.041.18165227
CTACTCG72050.041.1154437
GGCGCGT72050.041.0796216
TACTCGG71700.040.79631838
TGTAGTC73200.040.6166526
CGCGTGC73350.040.2734518
CCTGTAG73900.040.2127724