FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3146204
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG153930.4892562592889717No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG80670.25640422553655134No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC68650.21819945559792053No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC57000.1811707060317767No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG50210.1595891429799212No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG50000.15892167195769885No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT49520.15739602390690496No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG42330.13454308747938784No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT36770.11687099755769174No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG35980.1143600351407601No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC35130.11165836671747922No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34110.10841636460954217No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC34030.10816208993440984No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT32840.1043797541418166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48200.035.77946532
GCGGCAC49450.034.9556633
CGGCACC50700.033.96803334
CCGTCGC49350.033.35913545
CGCCCTA11900.033.19643861
CCCGTCG50200.032.4987144
TCGCCGG51550.031.92839648
GCACCCC54100.031.85259436
TCGTTTG16500.030.54034217
GGCACCC56900.030.45822735
CCCCCGT53150.030.4048642
CGTACGT1950.030.0993819
CCCCCGA58100.030.06890719
AGGACCG7300.029.70870870
CACCCCC59250.029.09652937
GTTCTCG17400.028.75727713
ACCCGCG61050.028.59488725
CTCGTTT17800.028.30446416
GATAGGC21900.027.90706340
CCCCGAC63000.027.68201820