FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3230764
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC122870.3803125205059856No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC107900.33397673120042193No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA78610.24331706060857433No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61710.1910074521073034No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC47580.1472716670112704No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC38700.1197859082248038No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36000.11142875183702677No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC34920.10808588928191598No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34320.10622874341796554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT56200.053.461449
TACGGCC5750.052.792677
CCGAACT9550.052.2352467
TGGCGCG59300.051.06120315
GTCTACG6250.050.7651564
CGCGGTG59500.050.61230510
GCCGGTT10100.049.1698653
TCTACGG6300.048.1725925
CTACGGC6700.045.302236
CGGTTCA11200.044.6486055
CGGCATA2250.044.600081
CCGGTTC11400.043.2602584
ACTCTTC27600.042.859095
CTACTCG72000.042.72056637
TACTCGG72500.042.54015438
GTAGTCC73200.042.30689627
TGTAGTC73550.042.28303526
TAGTCCC73250.042.1016928
GGCGCGT73900.042.04926716
GGGGTAG13500.041.7805331