FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3193950
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC122910.38482130277556004No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC106280.3327541132453545No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA77720.2433350553390003No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT60320.188857057875045No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC45400.1421437405094006No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC37780.11828613472346156No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG36020.11277571658917641No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC35760.11196167754661157No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34060.10663911457599523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT57950.054.004939
CGCGGTG60650.051.60075410
TGGCGCG61700.050.99984415
TACGGCC5550.049.7374347
CCGAACT8700.049.09367467
TCTACGG5950.047.552845
GCCGGTT9000.046.770253
GTCTACG6050.046.193624
CGGTTCA9400.045.1499065
CTACGGC6300.043.816316
CGAACTT10100.043.78794568
CCGGTTC9750.043.172544
GGCGCGT74000.043.1289816
GGGGACT37600.042.9463961
GTAGTCC74200.042.76566327
CTACTCG75200.042.40410637
TAGTCCC75050.042.3900728
TACTCGG75550.042.36147338
TGTAGTC75450.042.2775926
CGTGCCT75600.042.10832220