FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138769
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG148460.4729879771337107No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG83730.2667606313175643No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC69740.22218901741415187No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC58180.18535929213013128No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50040.15942555823636592No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG46340.14763749737556348No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG46300.14751005887977103No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT44740.14253995754386514No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG44600.1420939228085915No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC39610.1261959704584823No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT38670.1232011658073595No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC36910.11759387199249131No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC36340.11577787342744879No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA33880.10794040593621257No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG33760.10755809044883519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA48700.035.082832
GCGGCAC50050.034.2182433
CGGCACC52050.032.71292534
CCGTCGC50850.031.18948245
TCGCCGG51500.030.99733248
GCACCCC56400.030.28037336
TATCGGA2500.030.2727438
AGGACCG6300.030.22063370
GTTCCGT3000.029.911411
CCCGTCG51850.029.89394244
GTGCCGT4700.029.3729061
GTTCTCG19000.028.78761713
CACCCCC59750.028.5384237
GGCACCC60200.028.47172535
GTTATCG2800.028.2582876
CCCCCGA60350.028.23051519
TTATCGG2700.028.0307667
GTAGCGT6050.027.86095410
CTCGTTT19950.027.76164416
TCGTTTG19550.027.6302217