FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3217382
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC114620.3562523815947251No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC96080.29862789062660267No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA82870.2575696637825412No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT54980.17088427796264166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN50690.15755045561888517No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC44860.1394301329466007No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC41210.12808550554457007No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC34660.10772733856284394No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA33780.10499219551797082No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG32290.1003611010442652No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT52350.051.843079
CGGTTCA9350.051.4974825
CCGAACT9700.050.70823367
GCCGGTT9750.049.384763
TGGCGCG56600.048.42815415
TCTACGG5200.047.621025
CGCGGTG58050.047.10806710
GTCTACG5600.045.447844
CGAACTT11400.044.8580668
CCGGTTC10900.044.4899754
GGGGACT34750.043.3935171
TCTGGTC31550.043.17439310
TACGGCC5900.043.142987
GGACTCT35100.043.113683
TGGTCCG9800.042.82172412
ACTCTTC33150.041.7073025
CCGACTC8050.041.015213
GTAGTCC68400.040.55051827
TAGTCCC68700.040.4375528
CGGGACT8450.040.427481