FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3516700
Sequences flagged as poor quality0
Sequence length35-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA178680.5080899707111781No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC116080.3300821793158359No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC88950.25293599112804616No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT61360.17448175846674438No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC49230.13998919441521882No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48500.13791338470725395No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC42450.12070975630562744No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA37990.1080274120624449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGTT10450.052.748893
GCGCGGT54400.051.8644649
CCGAACT10800.051.25197667
TGGCGCG57400.049.0937615
CGGTTCA11250.048.997865
CGCGGTG58800.048.2763910
CCGGTTC11550.047.725194
TCTGGTC44950.047.5934110
GGGGACT46900.047.2984051
ACTCTTC45900.047.0613635
GGACTCT49500.046.074693
TCTACGG4450.045.677435
CGAACTT12800.044.88030268
TTCTGGT47600.044.799039
GGGACTC50950.044.6377332
CTGGTCC50250.042.5050511
CGGGACT10050.042.4207571
GTAGTCC68700.041.32573727
CTTAGGC13500.040.84841518
CTACTCG69800.040.8406137