FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318087

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318087
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4227964
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG260600.6163723248353108No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA243850.5767551473948218No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC215750.5102928974797326No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC199860.47270979601529245No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT106780.2525565496773388No Hit
GGCGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG73950.17490688189398018No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC50050.1183784913968047No Hit
CGGGTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCG50050.1183784913968047No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA48200.11400286284367606No Hit
GGCGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC43490.10286274906787286No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA43460.10279179292917347No Hit
CGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC43160.10208223154217963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG51050.064.2700354
TCTACGG51750.063.333195
CTACGGC52700.062.1915136
TACGGCC53050.061.8492287
ACGGCCA56850.057.8365758
CGGCCAT57150.057.351679
GGGTCTA43300.057.1057782
GCCATAC57550.056.4055811
CCATACC58700.055.3639512
CGTCTAC11450.054.8934943
CATACCA60050.054.06178313
GCGCGGT99900.053.8293579
CCTGGTT84550.053.74921470
GGCCATA61900.053.06228310
CCGAACT15100.052.74014367
GCGTCTA10600.051.4761622
CGCGGTG105100.051.26464510
TGGCGCG105900.051.0350115
TACCACC64050.050.73647715
CGAACTT16200.050.43116468