FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4231323
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG189250.4472596395973552No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC154420.36494495929523696No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG145120.3429660179570314No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC123420.2916818215012184No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT92050.21754425270772287No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG84780.20036286523151273No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT80550.19036599191316758No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA69580.16444029444218747No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG64440.15229279353053407No Hit
GCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCTT60670.14338305064397117No Hit
GGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT59400.14038162532144202No Hit
TGTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCG59280.14009802607836838No Hit
GCCGGTGCAAGGAGGGGAGGAGGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCAGCTT57390.13563133799995886No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG56890.13444967448715212No Hit
GGGGCTGGGGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA52730.12461823406059995No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG52140.12322387111548799No Hit
GTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACG51580.12190040798114442No Hit
TGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGG51220.12104961025192357No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG50600.11958434749604319No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG50490.11932438152322572No Hit
GTCCAGGGAGGCGTGCACCGCAGGGGTGAACTCGGCGGGGAGGTGGGCGG50450.11922984844220116No Hit
TGCGGGAAGTAGGTCTTGGTGGTGGGGAAGGACAGGAACATCCTCTCCAG46990.11105273693357846No Hit
GGTGGTGGGGAAGGACAGGAACATCCTCTCCAGGGCCTCCGCACCATACT44400.10493171993723949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCCGT22300.051.6452570
GTTCCGT5550.038.299981
CGCGGCA57750.037.0014432
GCGGCAC60050.035.5324633
ATGGCCG26200.035.44032369
AGGACCG7950.034.94587770
CGGCACC61800.034.36750834
TCGCCGG59750.033.2464548
CCGTCGC60200.033.0050145
CCCGTCG61250.032.4272344
GCACCCC66850.032.1078136
CCCCCGA67350.031.95888119
CACCCCC68450.031.1631837
GGCACCC71000.030.31328635
TCAGGAT26200.030.2690570
CTTACCC17700.030.2505370
ACCCGCG72100.030.11544625
TAGTCCG3900.030.1093466
GTCGGTT3450.030.0454864
GAACGAC3250.029.7551179
Produced by FastQC (version 0.11.5)