FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166395
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG83320.2631383639754358No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG42480.13415887784057265No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG37550.11858912106670204No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC31800.1004296684399767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA24450.035.21116332
GCGGCAC26700.032.63862633
TCGCCGG25650.032.18929748
CGGCACC27900.031.24124334
ACCCGCG29050.030.68820225
CCGTCGC26250.030.62756345
CCCGTCG26350.030.50164644
AGTCCGC29700.029.8676477
TACGGGT7750.029.5093576
CAGTCCG28750.028.685126
CCGCGCG30550.028.61957427
CCCCCGA30550.028.5928819
CCCCGAC31150.028.37955520
CCCCCGT29050.028.37093542
GTACGGG8150.027.6358785
GTCCGCC32150.027.5915768
CCCGACC32550.027.16279821
TCCGCCC33200.027.1364339
GCACCCC32750.026.72960536
GTTCCGT3200.026.0877151
Produced by FastQC (version 0.11.5)