FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3166395
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC70390.22230328180786038No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC63100.19928025404284683No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA55110.17404651030588414No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT36250.050.6378569
TGGCGCG37600.048.26477415
CGCGGTG40200.045.66224310
CTACTCG43500.041.99522837
GGCGCGT44050.041.59073316
TACTCGG44100.041.34839238
CGCGTGC44600.041.08563618
CCGAACT6800.040.9002767
GCGTGCC46300.039.507919
CGAACTT7400.039.32980768
CGTGCCT46750.039.20667320
ACTCGGG46800.039.19114339
GCGCGTG47500.038.7194317
GTAGTCC48800.037.9452827
AGGATCG46700.037.92703259
TAGTCCC49050.037.54394528
CGGCATA2800.037.285061
GCCGGTT7500.036.941593
TGTAGTC50500.036.80000326
GATCGCT48550.036.79310661
Produced by FastQC (version 0.11.5)