FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3151326
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG81470.25852609346034017No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40900.12978663584789388No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG37330.11845807130077943No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC31930.10132242744800125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA23500.036.044932
GCGGCAC26100.032.59241533
CGGCACC26550.031.7835134
ACCCGCG27200.031.10362625
TCGCCGG24700.030.86942748
CCCGTCG25600.030.16714144
CCGTCGC25350.030.0620745
CCGCGCG29000.029.54535927
TATCGCG605.295434E-428.86838510
CAGTCCG28200.028.8674686
AGTCCGC29750.028.644297
CCCCCGA29050.028.62904219
CCCCGAC29750.028.30665820
CCCGACC31200.027.21846221
TCCGCCC31850.027.0819479
AACGGCG2850.026.88724949
CCCCCGT28400.026.69401442
GCACCCC32200.026.53942136
CGTACGT1057.3992123E-726.40233619
CCGATCG8600.026.37781770
Produced by FastQC (version 0.11.5)