FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318098

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318098
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148274
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG85470.2714820882807532No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG40990.130198324542273No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG38340.12178101397781768No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC31710.10072185584863325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA23850.038.14077832
GCGGCAC25950.035.32576833
CGGCACC26800.033.9478534
TCGCCGG26100.032.94973448
CCGTCGC26500.032.69009845
AGTCCGC29000.032.6184927
CCCGTCG27000.032.21563344
ACCCGCG28750.031.97150225
CAGTCCG27600.031.7622076
CCCCCGT28100.030.82136242
CCCCCGA30250.030.59356519
CCCCGAC30400.030.33196820
GTCCGCC31500.030.2497238
TCCGCCC32000.030.210199
CCGCGCG30500.030.028627
CCCGACC31150.029.60448621
CGACCCG34050.026.98686823
GACCCGC34650.026.52420224
CGTACGT1456.675691E-1026.2947419
GCACCCC36150.025.74993136
Produced by FastQC (version 0.11.5)