FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148274
Sequences flagged as poor quality0
Sequence length35-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC71560.22729914867638587No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC63570.20192016323865078No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA52020.16523339455206248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT34050.047.398629
TGGCGCG36700.044.25991415
CGCGGTG36850.043.9850610
CGCGTGC40950.040.1840718
GGCGCGT41800.039.35754816
CCGAACT5550.039.31450367
CTACTCG42750.038.09916337
TACTCGG43500.037.7676338
GCGTGCC44150.037.1977919
GCGCGTG44150.037.18714517
CGAACTT5900.037.15867668
GTAGTCC44800.036.8467427
GGGGGGA55650.036.7665821
CGTGCCT44750.036.54826420
ACTCGGG46200.035.7939639
TAGTCCC46700.035.42526228
AGGATCG45550.034.65323659
TGTAGTC48200.034.3871126
CGGTTCA6650.034.373145
GATCGCT47300.033.98737761
Produced by FastQC (version 0.11.5)