FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3139543
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG85850.27344744123587417No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG42400.1350515027187078No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG37380.11906191442512494No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC32390.10316788144006946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA24550.033.93884732
TCGCCGG24400.032.24358448
CCGTCGC24950.031.37067445
TATCGCG450.00452277430.80560110
CGGCACC27300.030.64873534
GCGGCAC27150.030.4315233
CCCGTCG26300.030.15044444
ACCCGCG28550.029.64562625
CCCCGAC28500.029.56319220
AGTCCGC29400.029.2338877
CCCCCGA28950.028.86320319
CAGTCCG27750.028.8490246
CCCCCGT27400.028.6719542
CCGCGCG29550.028.41260127
CCCGACC30100.028.2230121
GTCCGCC30950.027.8818058
TCCGCCC32050.027.2492589
CCGATCG9350.026.441370
CGACCCG32600.026.0661523
GCACCCC33250.025.27872736
Produced by FastQC (version 0.11.5)