FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875916
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG284300.7335040284670772No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG117870.3041087577749363No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA83220.21471053552244165No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG75710.19533447061288223No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC70850.18279549918006477No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC54160.13973471045296132No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC54060.1394767069255371No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG52100.13441983778802225No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC49100.12667973196529544No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG46500.11997164025226553No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG45210.116643394748493No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC44670.11525017570040218No Hit
GCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCGC42250.10900649033673587No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA41350.10668445858991785No Hit
TGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA41160.10619425188781181No Hit
GGCCCCGAGAGAACCTCCCCCGGGCCCGACGGCGCGACCCGCCCGGGGCG41150.10616845153506937No Hit
GGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACGAG40450.1043624268430998No Hit
CGCTTCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA39270.10131798521949394No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA81650.037.6910432
GCGGCAC83800.036.68974733
CGGCACC85000.036.13983534
GCACCCC90400.034.30554236
TCGCCGG84400.034.0010448
CCGTCGC84200.033.99395845
CCCGTCG86300.033.19632344
CCCCCGA94150.033.0204719
GGCACCC93250.032.94973435
CACCCCC93700.032.9205637
CGTACGT2250.032.3120119
ACCCGCG97950.031.6978125
ATCCGTT1359.094947E-1230.7769242
TTTCGCG450.004554766730.7614255
CGCTTCC10950.030.458411
CCCCGAC103450.030.19013220
GACGTAT2100.029.83693344
GTTCCGT4200.029.7785361
GTGCCGT7350.029.7785321
CGCCCTA15250.029.4672761
Produced by FastQC (version 0.11.5)