FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3875916
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC159740.4121348347074601No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC142930.3687644417474476No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC81890.21127908860769945No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT79190.2043129933672453No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA67160.1732751690181108No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT55330.14275335172382475No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG52170.1346004402572192No Hit
GGGGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTC38900.10036337216802428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT78250.053.5450639
TGGCGCG81800.051.38715415
CGCGGTG82500.050.9963710
CGGCATA2150.048.5260241
CCGAACT11350.048.03690367
GCCGGTT12000.044.976553
TAGTCCC95450.044.70091228
GTAGTCC96000.044.47286627
TGTAGTC97100.044.10496526
CTACTCG97250.043.3919937
TACTCGG97950.043.095338
CCTGTAG99150.042.9317824
CGTGCCT98750.042.8324820
CGGTTCA13150.041.8341565
ACTCGGG102750.041.73751439
GCGTGCC102550.041.37551519
GGCGCGT103000.041.24703616
AGTCCCA103650.041.10667829
CGAACTT13750.041.09870568
CGCGTGC103600.040.8851318