FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3872576
Sequences flagged as poor quality0
Sequence length35-76
%GC60

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC156160.4032457981457304No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC141890.36639694095093295No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC78290.20216517377580193No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT78240.2020360607512932No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA62410.1611588771918227No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT53650.13853827529788956No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG50000.12911302450875078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT76500.052.4655889
CCGAACT11750.049.11221367
CGCGGTG82200.049.03792610
TGGCGCG82300.048.7637215
CGCGTAA451.6530321E-646.80399761
CGGTTCA12250.046.604895
CGGGTAG5400.043.9283561
CGAACTT14200.043.342468
GCCGGTT13500.043.310343
TACGGCC5650.042.8674437
GTAGTCC96800.042.20159527
TGTAGTC97700.042.0142126
TAGTCCC97550.041.88255728
CTACTCG96250.041.59995337
TACTCGG97400.040.9757438
GCCGAAC14050.040.9025966
CCTGTAG99600.040.88543324
CCGGTTC14400.040.6034474
ACTCGGG101700.039.90200439
CGTGCCT102050.039.6453120