FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942348
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG214070.43313421070309094No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG98520.19933845208795495No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG60370.12214842014362404No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG58860.11909319214268198No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52970.10717577960920599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGCAC71400.033.1398533
TCGCCGG70450.031.9720548
CGGCACC73900.031.65336434
CGCGGCA75450.031.35236432
CCGTCGC72300.031.05392845
CCCGTCG73150.030.72246644
GCACCCC81250.029.32145736
CCCCCGA84700.028.3301119
GGCACCC85650.027.56437735
CACCCCC87100.027.32425137
CGTACGT2950.026.91861319
ACCCGCG90150.026.80374725
CCCCCGT87350.026.1023742
TCGTTTG24900.025.36608117
AGGAGGG128150.025.27275870
CCCCGAC94550.025.23600220
CCATCGT11100.025.15275468
TTATCGG4950.025.0963757
CGTTACG3650.024.83933470
AGTCCGC98100.024.6230987
Produced by FastQC (version 0.11.5)