FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4942348
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC137520.278248314363942No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC113780.23021446486568733No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT72230.1461451115947319No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52510.10624504790030974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT72800.050.0010079
CCGAACT12500.046.77046667
TGGCGCG78350.046.5340115
CGCGGTG80500.045.21830410
GCCGGTT14050.043.2091033
CGGTTCA14450.042.0134285
CTACTCG90600.040.23294437
TAGTCCC91550.039.880628
CGAACTT15350.039.75124468
TACTCGG92250.039.53063638
CCGGTTC15750.038.9832764
TGTAGTC94350.038.936226
CGTGCCT95600.038.2013820
GGCGCGT97150.037.8496716
GTAGTCC98050.037.33425527
GCGTGCC98500.037.21194519
CCTGTAG98150.037.13047424
ACTCGGG99200.037.1210939
CGCGTGC101300.036.44819318
GTGGCGC103950.035.30875814