FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3736447
Sequences flagged as poor quality0
Sequence length35-76
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA175460.46959049599793595No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC129220.34583656612819613No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC100970.27022998051357344No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT57960.15512062662738157No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC46280.12386098344229156No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN44100.11802656373822512No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC40690.10890024667819455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT56800.051.3042349
CCGAACT10000.051.073867
GTCTACG8050.050.1146854
TCTACGG8250.049.3183865
TACGGCC8350.047.908927
TGGCGCG61850.047.4973215
GCCGGTT11000.047.332483
CGGTTCA11150.046.38715
CGCGGTG63650.046.10796710
GGGGACT44750.044.918041
TCTGGTC40100.043.86020310
CTACGGC9250.043.2446146
ACTCTTC41900.042.9573365
CGAACTT12550.042.82272368
CCGGTTC12400.042.2665634
GGACTCT46050.041.631323
GCCATAC9900.041.10471711
GGGACTC46600.040.5501942
CTACTCG74600.039.98060237
CTTAGGC12950.039.95553218