FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3446555
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA103710.30090916872065004No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76820.2228892328716646No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC61390.17811989073146955No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46250.13419196850188087No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT36130.10482931506968553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT35550.051.7874039
TGGCGCG38650.047.7187315
CGCGGTG39800.046.60383610
CCGAACT6550.044.5868467
GTACGCA10950.043.92697565
GCCGGTT6950.042.6583983
AGTACGC12200.040.5387264
CTACTCG45750.040.49321737
TACTCGG45850.040.26829538
TACGCAC11800.039.94157466
CGGCATA2200.039.3740431
GTAGTCC46600.039.3119527
TAGTCCC46850.039.2571728
CGGTTCA7500.039.0727355
TGTAGTC47250.039.0546626
GTTGATC13450.038.70589410
ACTCGGG48500.038.5131839
CGAACTT8200.038.0760868
ACGCACG11650.037.90791767
CGGGTAG4700.037.5980221