FastQCFastQC Report
Wed 8 May 2024
EGAF00005318128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3425805
Total Bases256.8 Mbp
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG198560.5796009988893122No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG71790.2095565859703048No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC63140.18430704608114007No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61730.18019122512810856No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGT56100.16375713153550772No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCAC47070.13739836330439123No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46930.1369897002310406No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT39820.11623545414873293No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCA38280.11174016034187587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)