FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3438701
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA100830.293221190211071No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC76790.22331107008140574No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC60980.1773344062190926No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46040.133887767502903No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT35320.10271320478285259No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA207.721014E-570.3860
GCGCGGT40400.050.515919
TGGCGCG43200.047.64073615
CGCGGTG44600.045.99069610
CGGCATA3500.041.6327321
GTACGCA10650.041.1706565
CTACTCG50550.041.105737
CCGAACT7050.040.93297667
TACTCGG51100.040.81339638
AGTACGC11500.040.53357364
TACGCAC10500.040.15594566
GTAGTCC52450.039.8738527
ACGCACG10300.039.7775567
GTTGATC12700.039.3597310
TAGTCCC53100.039.32926628
GGGGACT40150.039.316921
AAGTACG11950.039.26815863
TCTGGTC32350.038.5764410
ACTCGGG55000.038.56781439
CGGTTCA7350.037.991425
Produced by FastQC (version 0.11.5)