FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3433442
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG197610.5755448905209408No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG72150.21013898006723283No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC63480.18488735210904975No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63420.1847126003584741No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG53030.15445142221712205No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC48280.1406169086298822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN46630.1358112354890515No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41660.12133596548303423No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT35130.10231714996204976No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG35070.1021423982114741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG7650.035.8266870
CGCGGCA56750.034.04760732
GCGGCAC58600.033.09866333
CGGCACC59800.032.3869934
TCGCCGG57450.031.76362448
CCGTCGC59150.030.85098845
CCCGTCG60950.030.03735444
CGTACGT2950.029.25134719
CGATCTA3150.028.78565647
CCCCCGA68000.028.7807419
ACCCGCG67650.028.75913625
GCACCCC67750.028.71495836
AGTCCGC70100.028.4427477
CAGTCCG67200.028.4382066
CCCCGAC69350.028.32746720
GTACGGG11400.028.1397865
GAACGAC4300.028.0776849
TACGGGT11850.027.9456756
GGCACCC69700.027.79456535
TCCGCCC73200.027.5680779