FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4028030
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA231960.5758646286149806No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC166180.4125589928575507No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC123670.30702353259533816No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT71010.17628965027569307No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC61000.15143879266043203No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA50590.12559489378182387No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG48420.12020764492816588No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48140.11951251604382292No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA45420.11275983545306267No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT42700.1060071548623024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT75850.053.47979
GGGGACT52550.052.7005581
CCGAACT10550.051.39930767
CGCGGTG81550.049.9537210
ACTCTTC62200.049.8129845
TGGCGCG81600.049.74678815
TCTGGTC61800.048.73895310
CGGTTCA11000.048.7263575
GGACTCT65550.048.477563
GGGACTC64950.046.8491172
TTCTGGT65050.046.41029
GCCGGTT11550.045.8044173
CTGGTCC65900.045.8019111
TCTTCTG68950.044.587597
CGGGACT14150.044.447951
TCTACGG4550.044.0799945
TGTAGTC94350.043.6735726
GTAGTCC93800.043.64247527
CGAACTT12700.043.58146368
CTCTTCT71250.043.485866