FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4026584
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA225660.5604254127071483No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC168080.417425788211546No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC127470.3165710686775689No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT69980.17379495870445022No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC61340.1523375645460271No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC48810.12121937602692506No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA48400.12020114320227768No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG47410.11774248345495836No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA44790.11123572735599208No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT44570.11068935852325446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT76500.052.7319769
GGGGACT54050.051.0083661
GGACTCT65050.049.4089163
TCTGGTC61300.049.32736210
CGCGGTG82100.049.09301810
CGGTTCA13100.048.5940935
TGGCGCG82850.048.48036215
ACTCTTC64050.048.3438075
CCGAACT13050.048.25733667
TTCTGGT64700.046.7886739
GGGACTC64300.046.5960662
GCCGGTT13950.046.377183
CTGGTCC65450.046.14679311
CGGGACT13800.045.9096761
TCGCGTA551.2875353E-744.65968360
CGGGTAG5350.044.2452931
TCTTCTG70700.043.8944857
TGTAGTC93800.042.9533726
GTAGTCC93300.042.89398227
CGAACTT15200.042.52016468
Produced by FastQC (version 0.11.5)