FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4027912
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA210490.5225784475926981No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC156970.3897056340853524No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC118630.2945198405526238No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT68800.17080810107072847No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC58300.14474000425034111No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC47060.11683472727308838No Hit
CGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA46480.11539477525824794No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG46310.11497272035734644No Hit
GGGGGGAAGGCCCGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGT41580.10322966340873385No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA41450.10290691554333858No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT11100.052.9453667
GCGCGGT77500.052.3103569
GGGGACT52600.049.4606321
TGGCGCG84850.048.1831315
CGGTTCA12400.047.9809155
CGCGGTG85700.047.58770410
ACTCTTC58850.047.2575655
TCTGGTC56150.047.1273910
GCCGGTT12550.046.8532753
GGACTCT61000.046.836563
CGAACTT12900.046.55040768
GGGACTC60950.045.5260122
GTTGATC14700.045.17994310
TTCTGGT60000.044.6798979
CGGGACT12800.044.4103471
CTGGTCC60000.044.04573411
AGTACGC13850.043.70789764
GTAGTCC94200.043.42813527
TAGTCCC94800.043.19841428
TGTAGTC95600.042.88964526