FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3705565
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG234790.6336145769943315No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC123120.33225702423247194No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109450.2953665635334962No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG98740.26646408847233827No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG64010.17274018941780808No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62270.1680445492117936No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT51870.1399786537275692No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT46470.125405977226145No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA44430.11990074388116252No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG41990.11331605301755601No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACATTGCCCAGGAGCCTGAAGTTCTC38610.1041946369851831No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG38120.10287230152486868No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC37430.10101023730524224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA68550.036.10304632
GCGGCAC69850.035.4524133
CGGCACC71100.034.79941634
CCCGTCG69950.034.08490844
CCGTCGC69900.033.7792345
TCGCCGG69950.033.6405848
GCACCCC75250.033.12055636
CCCCCGA77800.032.0719819
CCCCCGT74450.031.78815742
ACCCGCG79550.031.36118325
GGCACCC79700.031.33481235
CACCCCC80150.031.1680337
CCCCGAC81550.030.73443820
AGTCCGC85200.029.577447
TCGCCGT3850.029.5369051
CAGTCCG82750.029.1254886
CCCGACC86700.029.0772821
CCGCGCG86300.029.02082827
TCCGCCC89750.028.4605059
AGGACCG7000.028.38872570