FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699128
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG236020.6380422629333183No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC122930.3323215633522279No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109810.2968537449907113No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG97060.26238616236042656No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG64660.17479795238229118No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG62920.1700941411056876No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT52560.1420875406311974No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT47610.1287060085512045No Hit
GTGCGCGTGCAGGTCGCTCAGGGCGGACAGCGCGTTGGGCATGTCGTCCA46100.12462396543185314No Hit
GGGGGTACGGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGG40490.10945822907452783No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC38170.10318648070572307No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACATTGCCCAGGAGCCTGAAGTTCTC37560.10153744341909769No Hit
GGGGGCAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG37370.10102380885441109No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA65400.037.7695332
GCGGCAC66100.037.33813533
CGGCACC68450.036.0253934
TCGCCGG66700.035.1791548
CGTACGT2250.035.11436519
CCGTCGC67250.034.90624645
CCCGTCG67850.034.87182644
GCACCCC74000.033.56351536
CCCCCGA74800.032.7436819
GGCACCC76550.032.42235635
ACCCGCG76250.032.29350725
CCCCCGT73200.032.08112742
CACCCCC77950.031.83742537
CCCCGAC78300.031.42401720
CCGCGCG81850.030.15977727
CCCGACC84500.029.17486221
AGTCCGC85100.029.1035777
CAGTCCG82600.028.7385486
CCCCGTC82600.028.5772543
TCCGCCC88800.028.277239