FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699128
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN108670.29377193760259174No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA106660.28833822457617037No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC67780.18323237260240793No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC49440.13365312041108066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT38400.047.529399
TGGCGCG40200.045.3950515
CGCGGTG42900.043.023610
CTACTCG44650.041.19626237
TACTCGG44500.041.13387738
CCGAACT6050.039.6561567
GTAGTCC46600.039.5820327
CGGCATA3300.038.7813151
ACTCGGG48300.038.63959539
TAGTCCC47650.038.59139328
TGTAGTC48750.037.88024526
TCTGGTC34700.037.86614210
GGGGACT42900.037.733171
AGGATCG47300.037.00475359
CGTGCCT48900.036.89260520
ACTCTTC36750.036.5006985
GATCGCT48550.036.27420461
GGACTCT39750.036.24443
CGCGTGC50350.036.13949618
GCGTGCC51050.035.59456619