FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3496117
Sequences flagged as poor quality0
Sequence length35-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC63450.1814870612167728No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC49500.14158565059464542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT30400.047.3389179
TGGCGCG32550.044.63974415
CGCGGTG35450.041.27956410
CTACTCG39350.037.41440637
TACTCGG39250.037.16173638
CGGCATA3000.037.035251
GGGGTAG18200.035.102091
GTAGTCC43300.034.4073327
CCGAACT6600.034.2855567
GGGGTAT13800.033.965751
TAGTCCC44100.033.86366728
GATCGCT43050.033.86144361
AGGATCG42900.033.85888759
ACTCGGG44150.033.67677739
TGTAGTC44500.033.3218626
CGGCATT6800.033.188761
CGAACTT7300.032.6154768
GCCGGTT6950.032.3585663
CGGCAAC6250.032.220671
CGGTATG2850.031.6748851
Produced by FastQC (version 0.11.5)