FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3540737
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138260.39048367613861185No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58540.16533281065495686No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG35910.10141956321522891No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN35760.1009959226002948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42550.035.30304332
CGTACGT2650.035.2756419
GCGGCAC43800.034.380133
CGGCACC45850.033.0793334
TCGCCGG43300.032.02579548
CCGTCGC43600.031.61186645
CTACCGG2250.031.48627570
GTACGTA2900.031.04602820
CCCGTCG44600.031.04503644
TCTAGCG4200.030.56637233
TACGTAG3100.030.1660721
ACCCGCG52750.029.3021525
CCCCCGA52050.029.26772119
CGATCTA3100.029.2087247
GCACCCC51650.029.10665336
GTTATCG4300.028.1690626
CACCCCC53650.028.09109137
CCCCGAC55750.027.64049720
GGCACCC55750.027.27243435
AGTCCGC58050.026.350747