FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3636378
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG141920.3902784584000893No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG60350.16596184445071444No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG37010.10177709798046297No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN36700.10092460134782469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42800.038.17643732
GCGGCAC45100.036.0047233
CGGCACC45950.035.3430834
ACGCGAC1751.8189894E-1234.83502670
TCGCCGG44750.034.17641448
CCGTCGC45100.033.8717845
CCCGTCG46150.033.3158144
GCACCCC54650.030.0461536
CCCCCGA55100.029.91998719
CACCCCC54950.029.89242637
ACCCGCG55850.029.5417925
GTTATCG2950.028.16546
GGCACCC58750.027.82575635
CCCCGAC60050.027.57241420
CGTCGCC56350.027.24501246
AGTCCGC61850.026.8675617
TCTAGCG4400.026.81120933
CCCCCGT57250.026.72039242
CCGCGCG62150.026.55196427
CAGTCCG59800.026.3991786
Produced by FastQC (version 0.11.5)