FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3598031
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG137630.38251476988386146No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG58410.16233879029947212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA42550.035.6285132
GCGGCAC44200.034.30470733
CCGTCGC43150.033.5561345
TCGCCGG43450.033.34916748
CGTACGT2000.032.8894219
CCCGTCG44750.032.8152344
CGGCACC46550.032.3569534
ACGCGAC1301.6907143E-731.25412870
CCCCCGA51800.029.87517219
GCACCCC52650.028.81428136
CACCCCC54050.027.94883537
ACCCGCG55050.027.88275525
GGCACCC56900.026.7186135
CGATCTA2500.026.46250347
CCCCGAC59250.026.12198620
AGTCCGC59950.026.0353187
CGTCGCC55900.025.90643746
GTTATCG4550.025.8608656
CAGTCCG58400.025.3633426
GTCGCCG57600.025.20756747
Produced by FastQC (version 0.11.5)