FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3850194
Sequences flagged as poor quality0
Sequence length35-76
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA108600.28206370899752065No Hit
GGGGGGCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGC62770.16303074598318942No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC61490.15970623817916707No Hit
GGGGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCC60250.15648562124402043No Hit
GGGGACTCTTCTGGTCCGCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA55520.14420052599946911No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53840.1398371095066898No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC50720.13173362173438533No Hit
GGGGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCTCCCACCCCTCCTCCCCGCGCCCCCGCCCCGGCG40120.1042025414823253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTA900.055.26168460
CGCGTAA1000.049.80053361
GCGCGGT34800.048.637419
ACTCTTC40200.045.9465945
TGGCGCG37700.045.0787915
CCGAACT6950.044.1972167
TGGTCCG13550.044.0877912
TCTGGTC41850.043.56196210
GGGGACT46150.042.8693161
GGACTCT45350.042.7767523
GGGACTC44750.042.126512
CGCGGTG40350.041.9480510
TTCTGGT44550.040.767189
CGGGACT8700.040.409861
CTGGTCC45300.040.31907311
TACGGCC4550.039.9904177
CGGTTCA7550.039.5611955
CTACTCG44250.038.73358537
TCTACGG4700.037.984165
TACTCGG44700.037.98324638