FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352120
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG167940.5009963843776476No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN134880.40237223011109385No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG61050.1821235516628283No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45550.13588415689175806No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG43180.12881400427192344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49900.038.00187732
GCGGCAC52150.036.45719533
CGGCACC53600.035.62419534
TCGCCGG51450.035.09290348
CCGTCGC51950.034.69164345
CCCGTCG54050.033.6262544
CCCCCGA58300.032.2538319
GCACCCC60450.031.85727936
ACCCGCG60600.031.24626525
CACCCCC62650.030.82471837
GGCACCC62800.030.70355435
CCCCCGT59500.030.19402542
CCCCGAC62850.029.99446320
CGTACGT2350.029.15007219
TCGTATG1950.028.51413240
CCGCGCG67700.028.3054127
AGTCCGC68600.027.8157867
CAGTCCG66050.027.5953356
ACGATCG500.00807788627.3571746
CGATCTA2700.027.23286847