FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3352120
Sequences flagged as poor quality0
Sequence length35-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132740.39598821044592675No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCC70630.21070248081810913No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGTCTCCTGCCGACAAGACCAACGTCA63540.18955168669379377No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC58990.1759781869384151No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTT37330.1113623617292937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGT38950.049.3245749
TGGCGCG41150.046.60167315
CGCGGTG43300.044.29038210
CCGAACT7450.040.20412467
CTACTCG48650.040.18292237
TACTCGG48750.040.13400738
CGGTTCA7300.039.8031585
GTAGTCC50250.039.21764827
ACTCGGG51350.038.5330139
TGTAGTC52000.037.9468126
CGGCATA3000.037.7827341
TAGTCCC52400.037.50550528
CGTGCCT52300.037.1448720
GCCGGTT8050.036.9429743
AGGATCG50200.035.84800359
CCGTACG1051.8189894E-1135.8106233
CCTGTAG55100.035.45668424
CGGCATT6400.035.4213141
GTCGTAC1750.035.159523
CGGGTAC3550.034.8317151
Produced by FastQC (version 0.11.5)