FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400199
Sequences flagged as poor quality0
Sequence length35-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG170730.5021176701716576No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN142400.4187990173516315No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG63820.1876948966810472No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG45040.13246283526346544No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG43080.12669846676620986No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG34350.10102349891873977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA49950.036.36089732
GCGGCAC52550.034.72646733
CGGCACC55350.032.87286434
CCGTCGC51700.032.8608445
TCGCCGG51850.032.7472348
CCCGTCG53100.032.03027344
GTGCCGT6050.031.86231
CCCCCGA58800.030.84409319
CGTACGT1450.030.67953719
GCACCCC60400.030.24271236
CACCCCC62800.029.28279537
ACCCGCG63450.028.6893325
GGCACCC63800.028.59546935
CCCCGAC63650.028.56407720
CCCCCGT60450.028.0793942
GTTCCGT3150.027.3195741
AGTCCGC68900.026.8745377
GTACGGG8900.026.8696825
CTCGTTT19600.026.84226416
TCGTTTG19850.026.68773717
Produced by FastQC (version 0.11.5)