FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005318174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005318174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3611221
Sequences flagged as poor quality0
Sequence length35-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG196240.5434173095471033No Hit
GGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCACG97800.27082252789292044No Hit
GGTGGGGTGAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTTGCCCAGGAGCCTGAAGTTCTC68670.19015729029045855No Hit
TGTGGGCCAGGGCATTAGCCACACCAGCCACCACTTTCTGATAGGCAGCC64680.17910839574758786No Hit
GGGTGCAGGAAGGGGAGGAGGGGCTGGGGGGAGGCCCAAGGGGCAAGAAG60590.16778258655451994No Hit
CTGGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCGCCGGGGCGGGGGCGCGGGGAGG55300.15313380155908488No Hit
GACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTG52610.14568479746877855No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA45460.12588539997967446No Hit
TGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC44250.12253473271228763No Hit
GGCGGGGAGGTGGGCGGCCAGGGTCACCAGCAGGCAGTGGCTTAGGAGCT41660.11536264327217857No Hit
AGGGGTGGGAGAGCGGTCGCGCCGTGGGAGGGGTGGCCCGGCCCCCCCAC41230.11417191027633035No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC40180.11126430644925914No Hit
CGTTTGAATATTTGCTACTACCACCAAGATCTGCACCTGCGGCGGCTCCACCCGGGCCCGCGCCCTAGGCTTCA40080.11098739179906188No Hit
GCTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCG39700.10993511612831229No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN37890.10492296095974188No Hit
GTGGCTTAGGAGCTTGAAGTTGACCGGGTCCACCCGAAGCTTGTGCGCGT37360.10345531331369641No Hit
GTGGGGTGGGGGTGGGGGGGGCGCGCCGGCGCCCGCCGGGCTCCCCGGGG36580.10129537904215777No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG350.001344326539.451666
AGGACCG8300.036.26859770
CGCGGCA62550.035.72016532
GCGGCAC64200.034.65374833
CGGCACC67350.032.98840334
TCGCCGG64150.031.9862448
CCCGTCG64000.031.93106544
CCGTCGC63900.031.83583545
GCACCCC71400.031.38177736
CACCCCC71500.031.15512837
TCGTTTG20450.031.0627617
GTTCCGT2400.030.3123261
CTCGTTT21100.030.26736816
GTTCTCG21450.030.09537113
CCCCCGA75200.030.08122419
GTACCGT703.746285E-529.6937081
GGCACCC77650.028.71027635
GTGCCGT5800.028.6697881
ACCCGCG80450.027.97518325
CGTGCGA8350.027.87932640
Produced by FastQC (version 0.11.5)